T-TAS (Total Thrombus formation Analysis System) is an automated microchip flow chamber system for the quantitative analysis of the thrombus formation process under blood flow conditions. T-TAS reproduces the in vivo thrombus formation process in an ex vivo system that uses the same substances that stimulate thrombus formation under physiological conditions. Therefore, T-TAS enables the ex vivo evaluation of overall hemostatic ability that more closely reflects the in vivo condition than other tests. Two types of T-TAS systems have been developed: T-TAS 01 for clinical use, equipped with two measuring channels and animated operation guide for efficient measurement, and T-TAS PLUS for research use, having additional functions of user-selectable flow rates and real time observation of thrombus formation with a CCD camera.
Advantages of T-TAS
Single-use microchip produced by a precision injection molding technique Requires only small-volume whole blood samples (approx. 320 μl) Simple operation controlled by a dedicated computer system
Our concept and mission
Studies using T-TAS have shown that considerable variability exists in both thrombogenicity and sensitivity to antithrombotic agents, which collectively result in large inter-individual variability with regard to thrombus formation among patients receiving antithrombotic therapy.
We believe that the measurement of thrombus formation under flow conditions using T-TAS will more directly reflect the potential for thrombotic and bleeding events. Thus, the application of T-TAS will aid in the optimization of therapeutic strategies in the clinical setting.
Why measure “under blood flow conditions”?
Blood flow is well recognized as a key factor that influences the thrombus formation process. Under arterial flow conditions, white thrombi comprising of numerous activated platelets and fibrin fibers are formed, whereas red thrombi form in the venous circulation predominantly consist of fibrin and red blood cells. The composition of thrombi affects the efficacy of antithrombotic agents. As analyzed by T-TAS, the efficacies of antithrombotic agents, including antiplatelet, anticoagulant, and thrombolytic agents, differ when measured under arterial and venous flow conditions. Using T-TAS, the thrombus formation process and effects of antithrombotic agents can be easily quantified and analyzed under shear stresses that simulate arterial or venous blood flow conditions.
thrombi formed under flow condition
thrombi formed under non-flow condition
SEM images of white thrombi formed inside the AR-Chip under arterial shear condition (left) and red blood clotting formed under static condition (right).