AR Chip for T-TAS01 (RUO)
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AR Chip for T-TAS®01 | |
FOR RESEARCH USE ONLY |
BACKGROUND Vascular haemostasis is achieved by complex physical and biochemical reactions of platelets and coagulation factors at the site of injury. Under blood flow conditions, platelet activation and coagulation processes are dynamically affected by platelets, coagulation factors, and their inhibitors. |
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WHAT is the AR Chip? AR chip has a 80 μm thick flow chamber coated with collagen and tissue thromboplastin, and mimics in vivo blood flow with 600/s shear stress, which represents shear stresses in large arteries. |
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HOW does it work? Whole blood is perfused at a constant flow rate at 37°C through the flow chamber pre-coated with tissue thromboplastin and collagen. The flow pressure changes are monitored by the pressure transducer located upstream in the chamber. Thrombus formation within the flow chamber increases flow resistance causing the pressure to increase. OST (time to 10 kPa: T10) is the lag time for the flow pressure to reach 10 kPa due to partial occlusion of the capillary. OT (occlusion time) is the lag time for the flow pressure to increase by 60 kPa from baseline owing to near complete occlusion of the capillary by . The area under the curve (AUC) is the area under the flow pressure vs. time curve and is related to overall thrombus formation. |
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A distinct advantage of using a flow chamber system for the measurement of thrombus formation is the correlation with the in vivo thrombus formation process. Scanning electron microscopic (SEM) analyses of thrombi inside AR chip showed that thrombi formed within the microchip capillaries under flow condition were tightly packed and contained numerous activated platelets. In contrast, thrombi formed under static condition was mainly composed of erythrocytes surrounded by fibrin fibers. |
SEM analysis of thrombi with / without shear stress |
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AR CHIP ASSAY SYSTEM
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AR Chip for T-TAS®01 | Reservoir | Cap&Over Cap | CaCTI Reagent for T-TAS®01 | Citrate Tube | T-TAS®01 Total Thrombus formation Analysis System Instrument |
REF#19001 | REF#19003 | REF#19004 | REF#18001 |
PERFORMANCE Dabigatran (factor IIa inhibitor) or Rivaroxaban (factor Xa inhibitor) was spiked to healthy blood in the presense or absence of aspirin and AR-C66096 (P2Y12 antagonist). AUC and OT varied in dose-dependent manner. |
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[Source]: K Hosokawa, et. Al: PLoS One 9(1):e86491 (2014),Comparative Evaluation of Direct Thrombin and Factor Xa Inhibitors with Antiplatelet Agents under Flow and Static Conditions: An In Vitro Flow Chamber Model. |
PRODUCT INFORMATION
Required but not provided in AR chip package
T-TAS®01 Total Thrombus formation Analysis System Instrument |
AR Chip & HD Chip Reservoir set for T-TAS®01 |
CaCTI Reagent for T-TAS®01 |
Citrate tubes (BD Life Sciences BD Vacutainer® REF#368273) |
Physical specifications of T-TAS® 01 Instrument
Dimensions |
(L x W x H) 36 x 32 x 24.7 cm |
Weight |
6.0 kg |
Operating Conditions |
Temperature: 20-30 °C Relative Humidity: 20-80% |
ORDERING INFORMATION
ITEM |
REF |
T-TAS ®01 Total Thrombus formation Analysis System Instrument |
18001 |
AR Chip for T-TAS®01 (20 Chips) |
19001 |
AR Chip & HD Chip Reservoir set for T-TAS®01(100 sets) |
19003 |
CaCTI Reagent for T-TAS®01 (20 assays) |
19004 |
REFERENCES
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Note: Most of above studies were performed by T-TAS PLUS. |
IMPORTANT NOTES The T-TAS 01 AR chip is FOR RESEARCH USE ONLY (RUO). Not for medical, diagnostic nor other use. |