WHAT’S T-TAS

T-TAS® (Total Thrombus-formation Analysis System) is an automated microchip flow chamber system for the quantitative analysis of the thrombus formation process under blood flow conditions. Using a precision injection molding technique, we developed two types of single-use microchip, “PL-chip” and “AR-chip” for evaluating “platelet specific” and “comprehensive” thrombus formation, respectively, under variable blood flow conditions.

PL-chip

PL-chip

coated with collagen
For quantitative evaluation of platelet thrombus formation under arterial flow conditions.
AR-chip

AR-chip

coated with collagen and tissue thromboplastin
For quantitative evaluation of white thrombus formation mediated by the activation of both coagulation and platelets.

T-TAS® is designed as Research purpose only (NOT IVD)

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Advantages of T-TAS®

  1. Single-use microchip produced by a precision injection molding technique
  2. Requires only small-volume whole blood samples (approx. 350 μl)
  3. Easy setting of the optional wall shear rates
  4. Dual-monitoring system (by flow pressure waveform and real-time video imaging )
  5. Simple operation controlled by a computer interlocking system 

Software Measurement control screen of T-TAS®

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Our concept and mission

Studies using T-TAS® have shown that considerable variability exists in both thrombogenicity and sensitivity to antithrombotic agents, which collectively result in large inter-individual variability with regard to thrombus formation among patients receiving antithrombotic therapy.

We believe that the direct measurement of thrombus formation under flow conditions using T-TAS® will more directly reflect thrombotic and bleeding events. Thus, the application of T-TAS® will aid in the optimization of therapeutic strategies in the clinical setting. 

Why measure “under blood flow conditions”?

Blood flow is well recognized as a key factor that influences the thrombus formation process. Under arterial flow conditions, white thrombi comprising of numerous activated platelets and fibrin fibers are formed, whereas red thrombi form under venous circulation predominantly consist of fibrin and red blood cells. The composition of thrombi affects the efficacy of antithrombotic agents.

As analyzed by T-TAS®, the efficacies of antithrombotic agents, including antiplatelet, anticoagulant, and thrombolytic agents, differ when measured under arterial and venous flow conditions.

Using T-TAS®, the thrombus formation process and effects of antithrombotic agents can be easily quantitated and analyzed under shear conditions that simulate arterial and venous blood flows.

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thrombi formed under flow condition
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thrombi formed under non-flow condition

 

SEM images of white thrombi formed inside the AR-Chip under arterial shear condition (left) and red blood clotting formed under static condition (right).

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